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Faculty Profile

 

Prof. Dhrubajyoti Chattopadhyay
M.Sc, Ph.D, FNA.Sc, FA.Sc

Guha Professor & Pro-Vice Chancellor (Academic Affairs),Calcutta University

Email:dhrubajyotic@gmail.com

  Educational Qualification

B.Sc (Hons) Chemistry – Presidency College, Calcutta, Calcutta University

M.Sc (Biochemistry) – Calcutta University

Ph.D (Biochemistry) – Bose Institute, Calcutta University

  Important Publications
1. Basak, S., Raha, T., Chattopadhyay, D., Majumdar, A., Shaila, M.S and Chattopadhyay, D.J. Leader RNA biding ability of Chandipura virus P protein is regulated by its phosphorylation status : a possible role in genome Transcription-replication switch. Virology. 307, 372-385 (2003). Impact Factor : 3.765

2. Mitra, A., Bhattacharyya, P., Chakroborty, K., Chattopadhyay, D.J. and Chakraborty, A. Physico-chemical properties, heavy metals and their relations in cultivated landfill soil dumped with municipal solid wastes. Archives of Agronomy and Soil Science. 49, 163-170 (2003). Impact Factor : 1.413

3. Misra, A., Chattopadhyay, R., Banerjee, S., Chattopadhyay, D.J. and Indu B. Chatterjee. Black Tea prevents Cigarette smoke-Induced Oxidative damage of proteins in Guinea Pigs. J. Nutrition, 113, 2622-2628. (2003). Impact Factor : 3.689

4. Goswami, A., Chatterjee, M., Mukherjee, A., Bhattacharya, A., Das, S. and Chattopadhyay, D.J. Study of human olfactory sensory system in response to various ororants in different dilutions on Eastern India population. J. Ecophysiol. Occup. Hlth. 3, 205-210 (2003)

5. Majumdar, A.; Bhattacharya, R.; Basak, S.; Shaila, MS.; Chattopadhyay, D.J.; Roy, S. P protein of Chandipura virus is an N-protein-specific Chaperone that acts at the nucleation stage.Biochemistry. 43, 2863-2870 (2004). Impact Factor : 4.63

6. Soumen Basak, Smarajit Polley, Mausumi Basu, Dhrubajyoti Chattopadhyay, Siddhartha Roy. Monomer and Dimer of Chandipura Virus Unphosphorylated P-protein Binds Leader RNA Differently: Implications for viral RNA synthesis. JMB. 339, 1089-1101 (2004). Impact Factor : 4.472

7. Sandipan Ganguly, Srikanta Ghosh, Dhrubajyoti Chattopadhyay and Pradeep Das. Antisense Molecular Beacon Strategy for In Situ Visualization of snRNA and Fibrillarin Protein Interaction in Giardia Lamblia. RNA Biology 1(1). 48-53, 2004.

8. Pal Chaudhuri, S., Sundaram, G., Bhattacharya, A., Ray, A., Chatterjee, I.B. and Chattopadhyay, D.J. Activation of S phase checkpoint by cigarette smoke extract in Schizosaccharomyces pombe. Yeast. 22, 1223-1238 (2005).Impact Factor: 2.619

9. Bhattacharya, R., Basak, S. and Chattopadhyay, D.J. Initiation of encapsidation as evidenced by deoxycholate-treated nucleocapsid protein in the Chandipura virus life cycle. Virology 349(1), 197-211(2006).Impact Factor: 3.765

10. Chattopadhyay, S., Sinha, N.K., Banerjee, S., Roy, D., Chattopadhyay, D.J. and Roy, S. A Small Cationic Protein from a Marine Turtle has B-defensin-like fold and Anti-bacterial and Anti-viral Activity, Proteins. 64(2)524-531, 2006. Impact Factor : 3.354

11. T. Das, R. Chattopadhyay ,S. Ghosh , S. Goswami, D.J. Chattopadhyay, B. N. Chakravarty and S. N. Kabir. Role of an Estrogen upregulated 64.0 kDa uterine fluid glycoprotein in improving fertility in women. Fertil. Steril. 87(2) 343-50,2007.Impact Factor : 3.168

12. S Banerjee, P Maity, S Mukherjee, A K.Sil, K Panda, D.J. Chattopadhyay and I B. Chatterjee. Journal of Inflammation,:3, 2007.Impact Factor: 3.19

13. A.Ghosh, B.Maity, K.Chakrabarti and D. J. Chattopadhyay. Bacterial Diversity of East Calcutta.Wet Land Area: Possible identification of potential bacterial population for different biotechnological uses. Microbial Ecology, 54(3):452-9,May 20, 2007. Impact Factor : 2.58

14. S. Basak, A. Mondal, S. Polley, S. Mukhopadhyay, D.J. Chattopadhyay. Reviewing: Chandipura: A Vesiculovirus in Human Epidemics. Bioscience Reports, 27; 275-298, Jul 4, 2007. Impact Factor: 3.115

15. A Ghosh, S Basu, H. Datta, D J Chattopadhyay. Evaluation of PCR Based Ribosomal DNA Sequencing Technique for the Diagnosis of Mycotic Keratitis. American Journal of Ophthalmology, 144(3):396-403, Jul11, 2007. Impact Factor: 2.628

16. S. Basak and D.J.Chattopadhyay. Chandipura virus (Rhabdoviridae).Encyclopedia of Virology, 3rd edition, Ed Brian Mahy and Marc Van Regenmortel, Elsevier Ltd,Oxford,UK.

17. Banerjee P, Talapatra SN, Mandal N, Sundaram G, Mukhopadhyay A, Chattopadhyay D, Banerjee SK. Genotoxicity study with special reference to DNA damage by comet assay in fission yeast, Schizosaccharomyces pombe exposed to drinking water. Food Chem Toxicol. Jan; 46(1):402-7, 2008. Impact Factor : 2.186

18. Sundaram, G., Palchaudhuri S ,Dixit S and Chattopadhyay D. MAPK mediated cell cycle regulation is associated with Cdc25 turnover in S.pombe after exposure to genotoxic stress. Cell Cycle.7 (3):1-8, 2008.Impact Factor: 3.1

19. Geetanjali Sundaram, Santanu Pal Chaudhuri, Sibapriya Chaudhuri, K. Sheelarani Dhrubajyoti Chattopadhyay, “Functional characterization of Sro1 a novel stress responsive gene in S.pombe”, (accepted in FEMS Yeast Research, 2008). Impact Factor : 2.812

20. M. Sinan, M. Panda, A. Ghosh, K. Dhara, P.E. Fanwick, D. J. Chattopadhyay, S. Goswami. Mild Synthesis of a Family of Planar Triazinium Cations via Proton Assisted 21. Cyclization of Pyridyl Containing Diazo Compounds and Studies on DNA Intercalation. Journal of the American Chemical Society. (Accepted, 13th Feb08).Impact Factor: 7.8

21. S Basu, A Ghosh, A Bera, MN Saha, D Chattopadhyay, K Chakrabarti. Thermodynamic characterization of a highly thermoactive extracellular pectate lyase from a new isolate Bacillus pumilus DKS1. Bioresource Technology (Accepted on 17th March2008).Nov, 99(17): 8088-94. Impact Factor : 3.1

22. A.Ghosh, Krishanu Chakrabarti, Dhrubajyoti Chattopadhyay. Degradation of raw feather by a novel high molecular weight extracellular protease from newly isolated Bacillus cereus DCUW. Industrial Microbiology & Biotechnology (Accepted on 4th April2008). Aug,35 (8) : 825-34. Impact Factor : 1.6

23. Dhrubajyoti Chattopadhyay. RNA Protein Interactions in Chandipur Virus Life Cycle.Arthopod Borne Viral Infections, Current Status and Research, p395 Edited by D.Raghunath and Durga Rao, Published by Tata McgrawHill, 2008

24. Rahul Gupta, Dhrubajyoti Chattopadhyay. Glutamate is the chemotaxis inducing factor in placental extracts. Amino Acid (2008, July, Accepted). Impact Factor 2.78

25. P. Bhattacharyya, A.Mitra, K.Chakrabarti , D. J. Chattopadhyay, A. Chakraborty & K. Kim .Effect of heavy metals on microbial biomass and activities in century old landfill soil. Environ Monit Assess. 136:299-306, 2008.


  Research interests

Development of Potent Anti-viral Agent(s) Against Chandipura Virus

  In recent years (2003, 2004 and 2007) Chandipura epidemic has been held responsible for hundreds of child deaths in the rural districts of Western India. This emerging pathogen causes death within 24 hours of infection leaving no opportunity for the patient to develop an immune response. The Chandipura virus has been suggested as “an emerging human pathogen” (M. Van Ranst; the Lancet, Volume 364, Issue 9437, Pages 821 – 822), and is also included in the ICMR 11th Five Year Plan report, and the annual report of the Indian Science Congress Association, 2004-2005, as a prime concern in the Indian subcontinent. Given the utmost urgency to develop anti-viral agents against this pathogen, we propose to chalk out strategies to restrict Chandipura outbreaks in future.

Leader RNA and Phosphoprotein (P) interaction plays a pivotal role in the Chandipura virus life cycle by dictating the course of molecular steps that ensue and follow either transcription or replication pathway. Thus understanding the basic molecular circuitries beneficiary for the viral multiplication process with special emphasis on the transcription-replication switch is of utmost importance. Using spectroscopic methods, interaction of full length as well as truncated and point mutated variants of Nucleoprotein (N) and Phosphoprotein (P) with both viral leader RNA and mini-genome length RNAs have been undertaken. This approach also includes use of fluorescently labelled proteins or RNA as the interacting species to determine the kinetics of the interactions.

Chandipura viral proteins, namely Nucleocapsid protein N and Phosphoprotein P, are involved in both homotypic as well as heterotypic interactions that result in N-N, P-P or N-P formation. As these interactions are crucial for the survival of the virus, deciphering the regions involved in the interaction would shed more light into the molecular details of the viral life cycle. Molecular Biological, and biochemical studies with both full length as well as truncated versions of N and P protein are already under way and use of more sophisticated biophysical and molecular biology techniques such as isothermal titration calorimetry, sedimentation equilibrium, confocal microscopy, and in-vivo FRET would enable us to locate the interaction domains more precisely and to determine their role in the production of mature viral particles, leading to functional domain mapping of these viral proteins.

Our group is also currently involved in developing and screening of potential antiviral compounds against this important human pathogen, Chandipura Virus. We are under the process of screening a number of natural and synthetic compounds for potent antiviral activity, and are further carrying out chemical modifications of promising compounds in order to minimize its cytotoxicity and maximize its antiviral activity. We plan to design and develop molecules to block leader RNA-P protein interaction in-vivo as it is one of the major molecular events during the viral life cycle and presents us a suitable target for rational drug designing. Other approaches to inhibit viral growth include development of antiviral defensins and to screen small molecule libraries to identify specific blockers of the leader RNA-P protein interaction. In addition to this we are also developing a high throughput system for rapid and reliable screening of chemical libraries.

 Microbial Bio-diversity in Wet-Land area: A metagenomic approach

Over the past 10 years we have seen increased interest in soil microbiology and ecology. This has been due to a better awareness that biological communities have a role in maintaining a sustainable biosphere and secondly too rapid advances in molecular biology. The challenge for a soil microbial ecologist is to identify the populations and guilds of microorganisms which have key functional roles in specific soil processes. PCR amplification of 16S rRNA genes (16S rDNA) using consensus bacterial primers and separation of the resultant PCR amplicons by denaturing gradient gel electrophoresis is a prime technique employed in this regard. The main aim of studying genetic diversity in wet-land soil using 16S rDNA methods should be to facilitate the isolation of novel organisms for the biotechnology industry.

In our laboratory, we have isolated different bacterial strains, from Eastern Wet-Land, Kolkata, India. Our next aim is to find out the non-cultivable microorganisms present in the above mentioned Wet-Land area, and also identification of the important bio-molecules that are synthesized by non-cultivable fraction of microbial consortia. Seasonal fluctuations of raw materials do not count and there are possibilities for genetic and environmental manipulation of microorganisms (bacteria and fungi) to give increased yields of desired enzymes in a way not possible with higher organisms. Moreover, the diversity of enzymes available from microorganism is very great. Lastly, Microbial enzymes present a wide spectrum of characteristics that makes them utilizable for quite specific applications.

Molecular Mechanism of Glutamic Acid induced Cell Migration in Neutrophils

Human placental extract has been found to be an efficacious against wound healing for a long time. When a wound is inflicted, neutrophils migrate to the wound site within minutes of injury to clear up the infection by engulfing the bacteria and also as the source of pro-inflammatory cytokines, which activate the surrounding fibroblasts and keratinocytes. HPLC analysis of the heat treated human placental extract revealed Glutamate to be the predominant free amino acid. We investigated the role of glutamate as a chemoattractant with particular attention to its ability to activate neutrophil responses.

  Lab Members

Arindam Mondal

PhD student (Senior research fellow)

arin.cal@gmail.com

Palash Ch. Maity

PhD student (Senior research fellow)

palashmaity6@yahoo.co.in

Arunava Roy

PhD student (Junior research fellow)

arunavaroy1@gmail.com

 

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